CacyBP/SIP protein reduces p53 stability by enhancing Mdm2 activity in p53 mutant glioma cells
نویسندگان
چکیده
Our previous studies have illustrated that CacyBP/SIP (Calcyclin-binding protein or Siah-1-interacting protein) promoted the proliferation of glioma cells. However, possible mechanism still needs to be clarified. In current study, we aimed uncover potential in regulating cell proliferation. We found decreased level p53, but not mRNA p53 mutant U251 line, whereas, wild-type U87 neither its nor regulated changes protein. Further investigation indicated interacted with and Mdm2 (Mouse double minute 2) promote ubiquitination subsequent proteasome-mediated degradation U251. Moreover, presence Mdm2, boosted a dose-dependent manner. On contrary, inhibition activity significantly increased stability p53. Finally, is inversely correlated human tissues. These observations suggest an underlying promotes by enhancing E3 ligase activity, which reveals novel pathway for regulation provides new therapeutic approach target CacyBP/SIP-induced
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ژورنال
عنوان ژورنال: Neoplasma
سال: 2021
ISSN: ['1338-4317', '0028-2685']
DOI: https://doi.org/10.4149/neo_2020_200414n399